Biomineralization of three calcium silicate-based cements after implantation in rat subcutaneous tissue
Talabani Ranjdar Mahmood, Garib Balkees Taha, Masaeli Reza, Zandsalimi Kavosh, Ketabat Farinaz,
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( Talabani Ranjdar Mahmood ) - University of Sulaimani Department of Conservative Dentistry
( Garib Balkees Taha ) - University of Sulaimani Department of Oral Diagnosis
( Masaeli Reza ) - Tehran University of Medical Sciences Department of Dental Biomaterial
( Zandsalimi Kavosh ) - University of Tehran Faculty of New Sciences and Technologies Department of Life Sciences Engineering
( Ketabat Farinaz ) - University of Saskatchewan Division of Biomedical Engineering
Abstract
Objectives: The aim of this study was to evaluate the dystrophic mineralization deposits from 3 calcium silicate-based cements (Micro-Mega mineral trioxide aggregate [MM-MTA], Biodentine [BD], and EndoSequence Root Repair Material [ESRRM] putty) over time after subcutaneous implantation into rats.
Materials and Methods: Forty-five silicon tubes containing the tested materials and 15 empty tubes (serving as a control group) were subcutaneously implanted into the backs of 15 Wistar rats. At 1, 4, and 8 weeks after implantation, the animals were euthanized (n = 5 animals/group), and the silicon tubes were removed with the surrounding tissues. Histopathological tissue sections were stained with von Kossa stain to assess mineralization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM/EDX) were also used to assess the chemical components of the surface precipitates deposited on the implant and the pattern of calcium and phosphorus distribution at the material-tissue interface. The calcium-to-phosphorus ratios were compared using the non-parametric Kruskal-Wallis test at a significance level of 5%.
Results: The von Kossa staining showed that both BD and ESRRM putty induced mineralization starting at week 1; this mineralization increased further until the end of the study. In contrast, MM-MTA induced dystrophic calcification later, from 4 weeks onward. SEM/EDX showed no statistically significant differences in the calcium- and phosphorus-rich areas among the 3 materials at any time point (p > 0.05).
Conclusions: After subcutaneous implantation, biomineralization of the 3-calcium silicate-based cements started early and increased over time, and all 3 tested cements generated calcium- and phosphorus-containing surface precipitates.
키워드
Biodentine; Biomineralization; EndoSequence
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